Regulatory

Part:BBa_K4235004

Designed by: Maulik Masaliya   Group: iGEM22_Stony_Brook   (2022-09-12)


pC promoter

This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid BBa_K4235002.

This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//promoter
Parameters
chassisSpodoptera Frugiperda